Construction of a p-nitrophenolate-sensitive membrane electrode and its application to an enzyme assay.
نویسندگان
چکیده
A p-nitrophenolate-sensitive membrane electrode was constructed by using tetraheptylammonium chloride as an ion-exchanger and o-nitrophenyl phenyl ether as a membrane solvent. The electrode responded to p-nitrophenolate down to 1 ,ƒÊM in a buffer solution of 0.1 M Na2CO3NaHCO3 (pH 10). The selectivity coefficients of the electrode to typical anions increased in the following order: Cl-<NO3< SCN-•¬p-nitrophenolate <ClO4. The method was applied to determine the enzyme activity of alkaline phosphatase.
منابع مشابه
High-Sensitive and Selective Liquid Membrane Electrode for Direct Determination of Trace Amounts of Chromium
N-(2H-[1,2,4]thiadiazolo[2,3-a]pyridine-2-ylidene)benzamide was used as a selective sensing material in the PVC membrane to construction of a new chromium ion selective electrode. This liquid membrane electrode worked well with a Nernstian response of 19.7 ±0.4 mV decade-1 of Cr(III) in a wide dynamic concentration range of 8.0× 10-6-1.0×10-1 M. The electrode had relatively short response time ...
متن کاملConstruction of an Ion-Selective Eelectrode for Determination of permanganate Ion
A PVC ion-selective membrane electrode based on Cd-tetraphenyl porphyrine as a carrier was prepared fordetermination of MnO4- ion. This electrode revealed good selectivity toward permanganate ion over a widevariety of anions. Effects of some experimental parameters such as nature of the plasticizer, the amounts ofcarrier and additive, and concentration of the internal solution on the potential ...
متن کاملApplication of a dioxime-PVC electrode to potentiometric studies of Cr(III) ion
A PVC membrane Cr(III) ion selective electrode has been constructed using 2H-1,4-benzothioazine-2,3(4H)dione dioxime (BTD) as membrane carrier. The influence of membrane composition on the electroderesponse was studied. The electrode exhibits a Nernstian response over a Cr(III) concentration range of 1.0 x10 6 to 1.0>< 10-1 M (r = 0.99) with a slope of 19.5 ± 0.5 mV per decade of concentration,...
متن کاملConstruction of S?-Selective Electrode and Thermodynamic Study of the Ternary Aqueous Mixed Electrolyte System (SrCl2, KG, H20) Using Potentiometric Method
In order to preparing strontium ion selective electrode 6-(4- nitropheny1)-2-pheny14, 4- dipropy1-3, 5-diaza-bicyclo [3, 1, 0] hex-2-ene (NPDBH) was used as an ionophore. This electrode responds to Sr (II)ion with a sensitivity of 28.2 ± 0.5 mV/decade over the range 1.0 x 10-6 to 1.0 Mat pH 3.0-10.0. Thelimit of detection was 2.4 x 10-7 M. The influences of membrane composition, the pH of the t...
متن کاملCloning, Expression and Purification of Truncated Chlamydia Trachomatis Outer Membrane Protein 2 (Omp2) and its Application in an ELISA Assay
Background: Although a simple and direct method does not exist for the detection of chlamydial infections, there are situations in which reliable serological tests, with sensi-tivity related to a specific antigen, could be helpful. Objective: The aim of this study was to clone the first 1100 bp of the C. trachomatis outer membrane protein 2 (omp2) gene in order to prepare a recombinant protein ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Chemical & pharmaceutical bulletin
دوره 35 9 شماره
صفحات -
تاریخ انتشار 1987